Valproic acid induces apoptosis dependent of Yca1p at concentrations that mildly affect the proliferation of yeast

Abstract
Valproic acid (VPA) inhibited the growth of yeast in a dose‐dependent manner with complete inhibition attained at 100 mM. When cells were exposed to 25 mM VPA, the wild‐type died showing apoptotic markers, while yca1Δ deleted of YCA1 encoding yeast caspase 1 survived. On the other hand, when cells were exposed to 50 mM VPA, both the wild‐type and yca1Δ died showing morphological features similar to those of the autophagic death of cdc28 which was also independent of YCA1. Thus, these results suggested that yeast cells die via YCA1‐dependent apoptosis when their proliferative activity is mildly impaired.