Molecular cloning of the cDNA for human erythrocyte beta-spectrin
Open Access
- 1 July 1988
- journal article
- Published by American Society of Hematology in Blood
- Vol. 72 (1) , 328-334
- https://doi.org/10.1182/blood.v72.1.328.328
Abstract
Overlapping cDNA clones, totaling 3.3 kilobases (kb) in length, which encode over 50% of the human erythrocyte beta-spectrin subunit, were isolated by antibody screening of a lambda gt11 expression library constructed from human fetal liver mRNA. The amino acid sequence of the C-terminus of beta-spectrin was derived. The size of beta-spectrin mRNA in human erythroleukemia cells was found to be 7.5 kb. Erythrocyte beta- spectrin is encoded by a gene located on human chromosome 14, as determined by cDNA hybridization to human X mouse somatic cell hybrids.Keywords
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