A 1H‐NMR method for determining temperature in cell culture perfusion systems
- 1 January 1993
- journal article
- research article
- Published by Wiley in Magnetic Resonance in Medicine
- Vol. 29 (1) , 113-118
- https://doi.org/10.1002/mrm.1910290120
Abstract
This report describes a noninvasive 1H-NMR method for measuring absolute temperatures (±0.2°C) in biological samples and, in particular, in cell culture perfusion systems, utilizing the linear temperature dependence of the water chemical shift relative to the temperature-independent shift of one of the components of the biological medium, e.g., pyruvate, acetate, or lactate. The effects of flow on temperature can be monitored and appropriate adjustments of the temperature controller can be made.Keywords
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