Phosphate Metabolism in the Culture Form of Trypanosoma cruzi*

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Abstract
SYNOPSIS. The uptake, distribution and turnover of phosphorus have been studied for the culture form of Trypanosona cruzi. Following exchange reactions, phosphorus was accumulated at an approximate rate of 1.9 μg/108 trypanosomes/hour in Krebs‐Ringerphosphate. Of 3.8 mg P/g trypanosomes (wet weight), 60% occurred in acid‐soluble, 13% in phospholipid, 22% in nucleic acid, and 5% in phosphoprotein fractions. Acid‐soluble and phosphoprotein fractions incorporated P32 more rapidly than phospholipid and nucleic acid fractions, the phosphoprotein fraction having the highest specific activity by 8 hours of incubation.