Bioorthogonal Turn‐On Probes for Imaging Small Molecules inside Living Cells
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- 30 March 2010
- journal article
- research article
- Published by Wiley in Angewandte Chemie International Edition in English
- Vol. 49 (16) , 2869-2872
- https://doi.org/10.1002/anie.200906120
Abstract
Glowing tags: A series of activatable (“turn‐on”) tetrazine‐conjugated fluorescent probes was developed, which react rapidly in an inverse‐electron‐demand [4+2] cycloaddition with strained dienophiles such as trans‐cyclooctene, thereby strongly increasing the fluorescence intensity (see picture). The novel turn‐on probes were applied for intracellular live‐cell imaging of a microtubuli‐binding trans‐cyclooctene modified taxol.Keywords
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