Effect of Inorganic Orthophosphate on in Vitro Activity of NADH-Nitrate Reductase Isolated from 2-Row Barley Leaves
Open Access
- 28 February 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 83 (3) , 472-474
- https://doi.org/10.1104/pp.83.3.472
Abstract
Inorganic orthophosphate (25 millimolar in assay media; Pi) was found to increase in vitro activity of NADH-nitrate reductase (NR) isolated from 2-row barley (Hordeum vulgare L.) leaves with a saturating concentration of nitrate (2 millimolar) but to decrease it with low nitrate levels (Km for nitrate as well as Vmax, whereas it does not alter the Km for NADH significantly. These results suggest that the interaction between a molybdenum site of the enzyme and Pi results in alteration of the properties of NR molecule.This publication has 10 references indexed in Scilit:
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