Site‐directed mutagenesis of bovine pancreatic ribonuclease: Lysine‐41 and aspartate‐121
- 9 April 1991
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 281 (1-2) , 275-277
- https://doi.org/10.1016/0014-5793(91)80410-5
Abstract
Chemical modification studies suggest that two residues of bovine pancreatic ribonuclease A (RNase A), Lys-41 and Asp-121, are important for catalysis. Three mutants of RNase A have been prepared, two point mutants with Lys-41 altered to Arg-41 and Asp-121 altered to Glu-121, and a double mutant where both residues are altered. The Lys-41 Arg mutant has ca. 2% the calatylic activity (kcalKm ) of the native protein, while the Asp-121 Glu mutant has ca. 17% the catalytic activity of the native protein. The double mutant has catalytic activity comparable to the Lys-41 Arg mutant.Keywords
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