Pasteurization of C1 Inactivator in the Presence of Citrate Salts1,2

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Abstract
Conditions were determined under which the complement C.hivin.1 inactivator (C.hivin.1-INA) can be pasteurized to reduce the risk of transfusion hepatitis associated with its use for replacement therapy in patients with genetic or acquired deficiencies. Recovery of 90% of the biological and immunological activity of a C.hivin.1-INA concentrate was achieved following heat treatment for 10 h at 60.degree. C in the presence of 3 M potassium citrate. Crossed immunoelectrophoresis in heparinized agarose was used to demonstrate the ability of the pasteurized C.hivin.1-INA to bind heparin and to form a prcipitation pattern with antibody which was almost indistinguishable from that of an unheated control. High pressure liquid chromatography and enhancement of the fluorescence of 1,8-anilinonaphthalene sulfonate were used to show that other proteins present in the concentrate were also stabilized.