Binding of Diazepam, Salicylic Acid and Digitoxin to Albumin Isolated from Fetal and Adult Serum

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Abstract
Albumin was isolated from pooled fetal serum obtained at normal delivery at term and from pooled adult plasma. Albumin isolation was carried out by means of PEG precipitation followed by ion exchange chromatography on DEAE-Sephadex A 50 and then on SP-Sephadex C 50. The binding of diazepam (1 μM), salicylic acid (2 mM) and digitoxin (6 nM) to albumin (40 g/l) was measured by equilibrium dialysis at 37°C. The unbound fraction (mean ± SD) for fetal and adult albumin of diazepam was 1.86 ± 0.24 and 1.82 ± 0.15% (NS), that of digitoxin was 3.18 ± 0.27 and 3.36 ± 0.04% (NS) and that of salicylic acid was 11.65 ± 0.99 and 9.47 ± 0.75% (p

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