Interfacing matrix-assisted laser desorption/ionization mass spectrometry with column and planar separations
- 30 March 2000
- journal article
- review article
- Published by Springer Nature in Analytical and Bioanalytical Chemistry
- Vol. 366 (6-7) , 691-700
- https://doi.org/10.1007/s002160051563
Abstract
This paper presents a critical review of off-line and on-line coupling of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to liquid column separations (e.g. HPLC, GPC and CE) and planar separations (e.g. PAGE and TLC). Off-line MALDI analysis of fractions collected from HPLC, GPC and CE or spots scraped and extracted from TLC and PAGE has already become a routine practice for many laboratories. MALDI has also been used to obtain mass spectra directly from TLC plates and PAGE. The direct analysis methods range from dot-blotted samples to two-dimensional scanning of the entire gels/plates. Various combinations of on-line coupling of MALDI with column separations are also reviewed. The review discusses the strengths and limitations associated with different off-line and on-line coupling approaches.Keywords
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