Carbohydrate components recognized by the cone‐specific monoclonal antibody CSA‐1 and by peanut agglutinin are associated with red and green‐sensitive cone photoreceptors

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Abstract
Previous investigations have demonstrated that the monoclonal antibody CSA‐1 and peanut agglutinin label specifically cone photoreceptor cells. In the present study, we compared the binding of CSA‐1 and peanut agglutinin to that of the monoclonal antibodies COS‐1 and OS‐2, which have been shown to recognize the red/green‐ and blue‐sensitive cone visual pigments, respectively. Using lectin and immunocytochemistry on serial semithin sections of the pig retina, we have demonstrated in the present study that both CSA‐1 and peanut agglutinin label specifically the red‐, and green‐sensitive, but not the blue‐sensitive cone cell outer segments. Peanut agglutinin does bind, however, to the cone matrix sheaths associated with all three types of cones. These observations support the idea that red‐, and green‐sensitive cone cells share some common molecular epitopes and may represent a differentiation line of cones, considerably different from that of blue‐sensitive cones.