American Ginseng. II. Analysis of Ginsenosides and Their Sapogenins in Biological Fluids

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Abstract
A GLC method was developed to assay individual ginsenosides and sapogenins in rabbit plasma and urine samples. A flavonoid, panasenoside, and a sterol, stigmasterol, were used as internal standards for ginsenosides and their sapogenins, respectively. Linear relationships of peak height ratio to weight ratio were obtained for ginsenosides (A1, 20-350 .mu.g; A2, 20-400 .mu.g; B2, 20-300 .mu.g; C, 20-500 .mu.g), and sapogenins (panaxadiol or panaxatriol, 10-200 .mu.g) in 0.1 ml of the silylation mixture. The GLC assay method developed was sensitive to 0.2 .mu.g of ginsenosides and 0.1 .mu.g of sapogenins.