An Isocratic HPLC Method for the Determination of Cephalosporins in Plasma

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Abstract
An isocratic reversed-phase liquid chromatographic method for the determination of 8 cephalosporins [antibacterial agents] in human plasma using UV detection at 254 nm is described. Plasma proteins were precipitated using acetonitrile prior to injection of a 10 .mu.l aliquot onto an octadecylsilane column. The mobile phases consisted of 6-11% acetonitrile in sodium dihydrogen phosphate (0.01M). The minimum detectable limit for each drug was < 1 .mu.g/ml of plasma. Possible interference from other drugs which might be administered concurrently is discussed. The reproducibility and precision of the method for cephalosporin assay are shown from the analysis of plasma containing 5-500 .mu.g/ml of plasma. The chromatographic behavior of the 8 cephalosporins was examined by varying mobile phase conditions.

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