Application of Ovine Luteinizing Hormone (LH) Radioimmunoassay in the Quantitation of LH in Different Mammalian Species

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Abstract
A sensitive double antibody radioimmunoassay was developed for measuring luteinzing hormone (LH) in various African mammalian species, using rabbit anti-ovine LH serum (GDN 15) and radioiodinated rat LH or ovine LH. Serum and pituitary homogenates from some African mammals as well as the domestic sheep, cow and horse and laboratory rat and hamster produced displacement curves parallel to that of the ovine LH standards. The species studied were: the cheetah (Acinonyx jubatus), spotted hyena (Crocuta crocuta), spring-hare (Pedetes capensis), porcupine (Hystrix africae-australis capensis), ground squirrel (Xerus inauris), bontebok (Damaliscus dorcas dorcas), blesbok (D. dorcas phillipsi), reedbuck (Redunca arundinum), sable (Hippotragus niger), roan antelope (H. equinus), impala (Aepyceros melampus(, tsessebe (D. lunatus), springbok (Antidorcas marsupialis), thar (Hmitragus jemlahicus), bush baby (Galago crassicaudatus), rhinoceros (Cerathotherium simum) and rock hyrax (Procavia capensis). The specificity of the assay was examined in detail for 1 sp., the rock hyrax. Radioimmunoassay and bioassay estimates of LH in hyrax pituitaries containing widely differing quantities of pituitary hormones were similar. In sexually active male hyrax mean plasma LH was 12.1 ng/ml and pituitary LH 194 .mu.g/gland, but in sexually quiescent hyrax mean plasma LH was 2.4 ng/ml and mean pituitary LH 76 .mu.g/gland. I.v. injection of 10 .mu.g of luteinizing hormone releasing hormone increased mean LH levels in hyrax from 0.9 ng/ml to 23.2 ng/ml by 30 min. Conversely, i.m. injection of 250 .mu.g testosterone induced a fall in LH levels in male hyrax from 1.7 ng/ml to 0.7 ng/ml 6 h after administration. Although the specificity of the assay for quantitating plasma LH in other species was not categorically established, there was a good correlation between plasma LH concentration and reproductive state in the bontebok, impala, spring-hare, thar, cheetah, domestic horse and laboratory rat, suggesting the potential use of the antserum in quantitating LH in a variety of mammalian species.

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