Immobilization of α‐chymotrypsin and trypsin on different agarose gels

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Abstract
Four preactivated agarose gels were evaluated with two proteases, α‐chymotrypsin and trypsin, for the amount of protein and active enzyme immobilized. CNBr‐Activated Sepharose 4B was found to be the best among the four gels tested since it gave the highest amount of active enzyme per g of gel for both α‐chymotrypsin (5.1 mg/g of gel) and trypsin (17.1 mg/g of gel). The lowest amount of active enzyme per g of gel was obtained with CNBr‐Activated Sepharose 6MB. The presence of spacer arms in Activated CH‐Sepharose 4B did not improve the coupling yields of the enzymes. When gels were lyophilized losses in enzyme activity (25–60%) were observed. In addition, CNBr‐Activated Sepharose 4B gel was resistant to abrasive treatment and lyophilization as illustrated by scanning electron micrographs.