Cl− transport in basolateral renal medullary vesicles: I. Cl− transport in intact vesicles

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Abstract
This paper provides the results of studies which characterized conductive36Cl− flux in basolaterally enriched membrane vesicles prepared from rabbit renal outer medulla. Conductive36Cl− uptake was studied under two different experimental conditions. In the first,36Cl− flux was driven by an inside positive voltage created with oppositely directed Cl− and gluconate gradients. In the second, an inwardly direct K+ gradient was used to drive36Cl− uptake. By these two methods, voltage-sensitive36Cl− uptake was shown to comprise about 45 and 65%, respectively, of the initial rates of total36Cl− flux. Separate paired studies demonstrated that the conductive36Cl− uptake was inhibited by the Cl− channel blocker diphenylamine-2-carboxylate (DPC) with an IC50 for DPC of 154 μm. The voltagedependent36Cl− uptake had an activation energy of 6.4 kcal/mole. This36Cl− conductance had an anion selectivity sequence of I−>Cl−≧NO 3 − ≫gluconate.

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