THE EFFECT OF VASOCONSTRICTOR SUBSTANCES IN SHED BLOOD ON PERFUSED ORGANS

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Abstract
The action of vasoconstrictor substances or vasotonins contained in shed blood on perfused organs was demonstrated by a comparison of the O2 consumption and the blood flow of kidneys and submaxillary glands of the cat in vivo with values found for the same organs in a perfusion apparatus. Both the Oa consumption and the rate of flow were considerably lower in the perfusion apparatus than in the animal. Inclusion of the ventilated and unventilated lung, liver or spleen in the perfusion circuit raised the blood flow through the kidney and the submaxillary gland in vitro to values found in the animal, indicating that the vasotonins had been removed from the perfusate. The addition of 0.02 mg. of ergotoxine to the perfusion fluid of isolated kidneys produced vasodilatation of short duration. Larger doses of the same drug (3 mg.) resulted in vasoconstriction, followed by an increase in blood flow lasting for several hrs. The perfusion pump used in these expts. permits parallel perfusion of 2 organs with pulsatile pressure and variable pulse rate. It consists of pyrex glass and is manufactured by Otto Hopf Inc., New York City.

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