BACTERIAL ADHESIVENESS AND INVASIVENESS IN CELL-CULTURE MONOLAYER .2. INVITRO INVASIVENESS OF 45 STRAINS BELONGING TO THE FAMILY ENTEROBACTERIACEAE

  • 1 January 1982
    • journal article
    • research article
    • Vol. 90  (6) , 409-413
Abstract
The invasive potential of 45 presumptive enteropathogenic and non-enteropathogenic bacterial strains belonging to the family Enterobacteriaceae was tested using the Sereny [guinea pig keratoconjunctivitis] test and [human epidermoid carcinoma] HEp-2 cell monolayers examined by a combined light optical method. All the presumptive enteropathogenic strains of Shigella dysenteriae, S. boydii, S. flexneri, S. sonnei and Salmonella typhimurium showed in vitro invasiveness in the Hep-2 cell culture test. Fourteen presumptive non-enteropathogenic strains of Escherichia coli showed no invasiveness in either test system. Two strains of S. flexneri and the 6 strains of S. typhimurium gave a negative result in the Sereny test, although they were invasive in HEp-2 cell cultures. Otherwise, there were correlative results between the cell monolayer test and the Sereny test. In the cell monolayer test, the different species of enteropathogenic bacteria showed considerable variation in invasive potential.