Amyloid β-peptide spin trapping II

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Abstract
SYNTHETIC β-amyloid peptides (Aβs) react with the spin trap phenyl-tert-butyl nitrone (PBN) to form products detectable by electron paramagnetic resonance (EPR) spectroscopy. At least two EPR-detectable products can be distinguished from the Aβ/PBN reaction, and peptide toxicity towards glutamine synthetase enzyme correlates with the type of PBN reaction product observed. We have reacted synthetic Aβ(25–35) peptide with [12C]-or [13C]PBN to demonstrate that the two products represent alternate pathways of spin adduct decomposition. Results indicate that the C=N bond of PBN is cleaved by Aβ in what we hypothesize is a radical addition-fragmentation reaction.

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