CONSISTENT ACTIVATION OF FETAL HEMOGLOBIN SYNTHESIS IN CULTURED ADULT BONE-MARROW CELLS
- 1 January 1978
- journal article
- research article
- Vol. 51 (4) , 671-679
Abstract
The production of fetal Hb (HbF) was investigated in [human] plasma clot cultures of adult bone marrow cells from normal donors and from individuals with homozygous HbS or HbC disease. Synthesis of .gamma. and .beta. chains was assessed by 35S-methionine labeling cultures and measurement of the radioactivity incorporated into the methionine-containing tryptic peptides of the .gamma. and .beta. subunits or by 3H-leucine labeling and measurement of the radioactivity incorporated into the globin chains of HbF0 and HbA0 isolated by ion-exchange chromatography. The cultures from all individuals responded with increased production of HbF. Cultured cells from subjects without a hemoglobinopathy produced an average of 8.2% .gamma. chains (range 3.1%-20.3%), while cultured cells from subjects homozygous for HbS or HbC produced an average of 16.6% .gamma. chains (range 12.2%-20.4%). HbF production was probab ly regularly enhanced in adult bone marrow cells triggered in vitro to clonal growth in the plasma clot culture system.This publication has 7 references indexed in Scilit:
- Hemoglobin F synthesis in vitro: evidence for control at the level of primitive erythroid stem cells.Proceedings of the National Academy of Sciences, 1977
- Characterization of an erythroid precursor cell of high proliferative capacity in normal human peripheral blood.Proceedings of the National Academy of Sciences, 1977
- HUMAN MARROW CELLS CAPABLE OF ERYTHROPOIETIC DIFFERENTIATION INVITRO - DEFINITION OF 3 ERYTHROID COLONY RESPONSES1977
- Hemoglobin switching in sheep and goats. VI. Commitment of erythroid colony-forming cells to the synthesis of betaC globin.The Journal of cell biology, 1976
- Erythropoietin sensitivity as a differentiation marker in the hemopoietic system: Studies of three erythropoietic colony responses in cultureJournal of Cellular Physiology, 1976
- Structural evidence for γ globin chain synthesis in adult bone marrow culturesBiochemical and Biophysical Research Communications, 1976
- Stimulation of fetal hemoglobin synthesis in bone marrow cultures from adult individuals.Proceedings of the National Academy of Sciences, 1976