The Separation of X- and Y-Spermatozoa with Regard to the Possible Clinical Application by Means of Artificial Insemination
- 24 April 2009
- journal article
- Published by Hindawi Limited in Andrologia
- Vol. 9 (3) , 289-292
- https://doi.org/10.1111/j.1439-0272.1977.tb01305.x
Abstract
With the sephadex gel-filtration method according to Steeno and Adimoelja (1974), X-spermatozoa have been successfully separated from the Y-spermatozoa in semen samples from 81 donors with the possibility of practical use with the aid of artificial insemination to promote pregnancy. A better quality of spermatozoa is obtained after this method of filtration which will support these spermatozoa in having the potency of fertilization. There is indeed a decrease in concentration of spermatozoa after filtration, but the samples still fulfil the minimal concentration required in artificial insemination. Mit der Sephadex-Gel-Filtration nach Steeno und Adimoelja konnten X-Spermatozoen erfolgreich von den Y-Spermatozoen bei 81 Probanden isoliert werden. Eine bessere Spermatozoenqualität wurde auf diese Weise insofern erzielt, als damit eine höhere Fertilisationsrate der Spermatozoen erreicht wird. Man findet zwar eine Reduzierung der Spermatozoendichte durch die Filtration, aber dennoch wird eine ausreichende Konzentration für die artifizielle Insemination erreicht.Keywords
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