Generation of Cytotoxic Lymphocytes in Vitro: Response of Immune Rat Spleen Cells to a Syngeneic Gross Virus-Induced Lymphoma in Mixed Lymphocyte-Tumor Culture
Open Access
- 1 May 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 116 (5) , 1367-1372
- https://doi.org/10.4049/jimmunol.116.5.1367
Abstract
Spleen cells from W/Fu rats 4 to 6 weeks after immunization with syngeneic Gross virus-induced lymphoma (C58NT)D cells usually lack detectable activity in a short-term 51Cr release assay. The results presented here demonstrate that these spleen cells retain the capacity to generate significant proliferative and cytotoxic activity upon re-exposure to mitomycin C-treated (C58NT)D cells in vitro. Optimal conditions were defined in W/Fu rats for this secondary immune response in vitro to the (C58NT)D cells. The cytotoxic response was observed to be quantitative, reproducible, and specific. Optimal generation occurred 5 days after initiation of cultures with a 30:1 responding cell:stimulating cell ratio. In vitro generated cytotoxic cells inhibit tumor growth in vivo when administered as a mixture with tumor cells.This publication has 0 references indexed in Scilit: