Esp1396I restriction-modification system: structural organization and mode of regulation
- 15 January 2003
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 31 (2) , 743-749
- https://doi.org/10.1093/nar/gkg135
Abstract
Esp1396I restriction-modification (RM) system recognizes an interrupted palindromic DNA sequence 5'-CCA(N)(5)TGG-3'. The Esp1396I RM system was found to reside on pEsp1396, a 5.6 kb plasmid naturally occurring in Enterobacter sp. strain RFL1396. The nucleotide sequence of the entire 5622 bp pEsp1396 plasmid was determined on both strands. Identified genes for DNA methyltransferase (esp1396IM) and restriction endonuclease (esp1396IR) are transcribed convergently. The restriction endonuclease gene is preceded by the small ORF (esp1396IC) that possesses a strong helix-turn-helix motif and resembles regulatory proteins found in PvuII, BamHI and few other RM systems. Gene regulation studies revealed that C.Esp1396I acts as both a repressor of methylase expression and an activator of regulatory protein and restriction endonuclease expression. Our data indicate that C protein from Esp1396I RM system activates the expression of the Enase gene, which is co-transcribed from the promoter of regulatory gene, by the mechanism of coupled translation.Keywords
This publication has 25 references indexed in Scilit:
- Transcriptional Analysis and Regulation of Expression of the Scr FI Restriction-Modification System of Lactococcus lactis subsp. cremoris UC503Journal of Bacteriology, 2001
- Structural organization and regulation of the plasmid-borne type II restriction-modification system Kpn2l from Klebsiella pneumoniae RFL2Nucleic Acids Research, 1999
- Horizontal gene transfer contributes to the wide distribution and evolution of type II restriction-modification systemsJournal of Molecular Evolution, 1996
- The eco72IC gene specifies a trans-acting factor which influences expression of both DNA methyltransferase and endonuclease from the Eco72I restriction-modification systemGene, 1995
- Sequence motifs characteristic for DNA [cytosine-N4] and DNA [adenine-N6] methyltransferases. Classification of all DNA methyltransferasesGene, 1995
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Translational control of phage f1 gene expression by differential activities of the gene V, VII, IX and VIII initiation sitesJournal of Molecular Biology, 1987
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- The origin of replication of plasmid p15A and comparative studies on the nucleotide sequences around the origin of related plasmidsCell, 1983
- A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powderAnalytical Biochemistry, 1982