MICROWAVE FIXATION OF CELLS IN TISSUE-CULTURE

  • 1 January 1980
    • journal article
    • research article
    • Vol. 55  (2) , 71-75
Abstract
Use of microwave irradiation was evaluated for the in situ fixation of [human diploid lung] cells grown in tissue culture prior to fluorescent antibody staining. The results show total retention of cell protein in the matrix with microwave fixation, while 40-50% of the protein is lost during conventional formaldehyde fixation. Fluorescent antibody staining of cells shows that this protein loss reflects cell loss. A further advantage of microwave fixation is that photometric quantitation of fluorescence appears possible by random scanning of fluorescent antibody stained populations.

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