DIFFERENTIATION OF SMOOTH AND NONSMOOTH COLONIES OF BRUCELLAE
- 1 February 1951
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 61 (2) , 239-240
- https://doi.org/10.1128/jb.61.2.239-240.1951
Abstract
Cultures are streaked on Albimi brucella agar modified to contain 2.5% agar, 1% glucose, and 5% glycerol, and incubated at 37[degree] C for 96 hrs. The plates are then flooded with 1:2,000 aqueous soln. of crystal violet for 15 sec., and the excess dye decanted into a disinfectant. Observed under a wide field microscope using obliquely transmitted light, smooth colonies appear light blue-green, rough colonies appear violet-red, and mucoid colonies appear blue-red to purple. Viable cells can be picked during 96 hrs. after flooding the colonies.Keywords
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