Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts
- 1 October 1988
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 7 (6) , 379-384
- https://doi.org/10.1007/bf00269517
Abstract
Transgenic rice plants have been regenerated by somatic embryogenesis from cell suspension derived protoplasts electroporated with plasmid carrying the NPTII gene under the control of the 35S promoter from cauliflower mosaic virus. Heat shock of protoplasts prior to electroporation maximised the throughput of kanamycin resistant colonies. Omission of kanamycin from the medium for plant regeneration was essential for the recovery of transgenic rice plants carrying the NPTII gene. This report of the production of kanamycin resistant transgenic rice plants establishes the use of protoplasts for rice genetic engineering.Keywords
This publication has 37 references indexed in Scilit:
- Genetically Transformed Maize Plants from ProtoplastsScience, 1988
- Evaluation of Selectable Markers for Obtaining Stable Transformants in the GramineaePlant Physiology, 1988
- Electroporation-mediated improvement of plant regeneration from colt cherry (Prunus avium × pseudocerasus) protoplastsPlant Science, 1988
- Transformation of Rice Protoplasts by Direct DNA UptakePublished by Springer Nature ,1988
- Stable transformation of maize after gene transfer by electroporationNature, 1986
- Chimeric vector construction for higher-plant transformationGene, 1985
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962