Effect of Uridine Dethiolation in the Anticodon Triplet of tRNA(Glu) on its Association with tRNA(Phe)
- 1 June 1988
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 5 (6) , 1259-1266
- https://doi.org/10.1080/07391102.1988.10506468
Abstract
The effect of U(34) dethiolation on the anticodon-anticodon association between E.coli tRNA(Glu) and yeast tRNA(Phe) has been studied by the temperature jump relaxation technique. An important destabilization upon replacement of the thioketo group of s2U(34) by a keto group, was revealed by a lowering of melting temperature of about 20° C. The measured kinetic parameters indicated that this destabilization effect was originated in an increase of dissociation and a decrease of association rate constants by a factor of 4 to 5. Modifications in both stacking interactions and flexibility in the anticodon loop would be responsible for this effect.This publication has 19 references indexed in Scilit:
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