EFFECTS OF INTENSITY AND FLUENCE UPON DNA SINGLE‐STRAND BREAKS INDUCED BY EXCIMER LASER RADIATION

Publisher Website
Google Scholar
Abstract
Abstract—A Xenon‐chloride excimer laser emitting energy at 308 nm was used to induce single‐strand breaks (SSBs, frank breaks plus alkali‐labile lesions as assayed by alkaline sucrose sedimentation techniques) in purified DNA fromBacillus subtilis. A fluence response study and a peak pulse intensity study were performed. At a pulse energy of 0.1 mJ/pulse, the radiation induced SSBs in a linear fashion (91 SSB/108Da per MJ/m2) to a maximum exprimental fluence of 1.28 MJ/m2. The pulse intensity study showed that there were no significant changes in DNA breakage (105 SSB/108Da) between 2.93 times 109and 5.86 times 1011W/m2(0.11 and 22.0 mJ/pulse) at a constant total fluence of 1.1 MJ/m2(27000 mJ dose). This study has verified and extended previous work by quantifying the yield of SSBs induced in DNA by this laser radiation.

This publication has 21 references indexed in Scilit: