Ceramide glycosylation and fatty acid hydroxylation influence serological reactivity inTrypanosoma cruziglycosphingolipids
Open Access
- 1 March 2005
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 244 (1) , 47-52
- https://doi.org/10.1016/j.femsle.2005.01.024
Abstract
Ceramide mono (CMH) or dihexoside (CDH) fractions from Trypanosoma cruzi (Dm28c clone) were identified as glucosyl and lactosylceramides containing non-hydroxylated fatty acids. The di-glycosylated form was much more efficiently recognized by sera from T. cruzi-immunized rabbits, indicating that glycosylation influences antigenicity. Fatty acid hydroxylation was also a determinant of serological reactivity, since an α-hydroxylated CMH, only present at the Y clone, was recognized by the hyperimmune sera. In summary, these data indicate that T. cruzi CMHs with non-hydroxylated fatty acids are unable to induce antibody responses in animal hosts, which is reverted by the addition of a sugar residue or an α-hydroxyl group.Keywords
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