Synthesis and Biological Activity of Polyethylene Glycol−Mouse Nerve Growth Factor Conjugate
- 31 August 1999
- journal article
- research article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 10 (6) , 932-937
- https://doi.org/10.1021/bc990001k
Abstract
Conjugation of poly(ethylene glycol) derivatives with therapeutic proteins is a promising approach for enhancing protein stability and, therefore, effectiveness. An N-hydroxysuccinimidyl ester of fluorescein-PEG 2000 was used for chemical modification of mouse nerve growth factor (mNGF), a dimeric protein with therapeutic potential for Alzheimer's disease. The mNGF-PEG2000-fluorescein conjugate was characterized by RP-HPLC, spectrofluorometry, and SDS−PAGE and was biologically active, as determined by two independent NGF-specific assays (enhancement of ChAT activity in fetal neurons and neurite outgrowth in PC12 cells). The conjugate was not detectable by a standard NGF ELISA, suggesting a fortuitous reduction in protein recognition by antibodies.Keywords
This publication has 10 references indexed in Scilit:
- Systemic Administration of a Nerve Growth Factor Conjugate Reverses Age-Related Cognitive Dysfunction and Prevents Cholinergic Neuron AtrophyJournal of Neuroscience, 1996
- Zero-Length Cross-linkersPublished by Elsevier ,1996
- Theoretical Studies on the Bioactive Conformation of Nerve Growth Factor Using VBMCA Novel Variable Basis Monte Carlo Simulated Annealing Algorithm for PeptidesJournal of the American Chemical Society, 1996
- Chemistry of polyethylene glycol conjugates with biologically active moleculesAdvanced Drug Delivery Reviews, 1995
- Flow Cytometric Analysis of Fluorescein-Labeled Nerve Growth Factor Binding to A875 Human Melanoma CellsExperimental Cell Research, 1994
- Nerve Growth Factor and Alzheimer's DiseaseReviews in the Neurosciences, 1994
- NGF and the Treatment of Alzheimer's DiseaseExperimental Neurology, 1993
- The carboxyl terminus of nerve growth factor is required for biological activity.Journal of Biological Chemistry, 1993
- Targeting of Liposomes to Cells Bearing Nerve Growth Factor Receptors Mediated by Biotinylated Nerve Growth FactorJournal of Neurochemistry, 1987
- Receptor Binding Activities of Biotinylated Derivatives of β‐Nerve Growth FactorJournal of Neurochemistry, 1986