RAPID EXTRACTION OF GRASS POLLEN ALLERGENS AND SEPARATION OF THEIR ACTIVE FRACTIONS

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Abstract
The immediate effects of nasal provocation tests with dry grass pollen, correlated with recent botanical evidence of almost instant release of allergens from the pollen wall, suggested that prolonged extraction of pollens was unnecessary and perhaps undesirable. It has now been shown that all the active allergens from undefatted dry grass pollens of the 12 major British species are extracted completely in 15 min. Gel chromatography of such rapid extracts has shown clear-cut separation of active from inactive material, as confirmed by skin and nasal provocation testing. It is probable that active fractions thus obtained contain the only pollen allergens of importance to clinical allergy. Further purification may produce specific allergens useful for the accurate standardisation of vaccines.