Improved Method for IsolatingPhytophthora lateralisfrom Soil

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Abstract
Recovery of P. lateralis from soil was improved by adding hymexazole to the baiting solution, then placing baits into a solid antibiotic medium. Port-Orford-cedar [Chamaecyparis lawsoniana] branchlet baits were floated over a soil sample in water amended with 25 .mu.g/ml hymexazol. Baits were transferred after 6 days to cornmeal agar containing 20 .mu.g/ml of pimaricin and 200 .mu.g/ml each of vancomycin and streptomycin (CMP). Hymexazol in the baiting solution limited colonization by Pythium isolates. No effect on growth or sporulation of P. lateralis was observed. Hymexazol and the antibacterial amendments prevented faster-growing contaminants from masking or inhibiting growth of P. lateralis on CMP. P. lateralis was successfully recovered from naturally infested soil diluted 16 times with uninfested soil.

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