EXTRACELLULAR PHOTOSYNTHETIC REACTIONS. II. IDENTIFICATION OF RADIOACTIVE MALATE

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Abstract
An illuminated cell-free enzymatic system derived from sugar beet and spinach leaves and consisting of chloroplast fragments, a malic enzyme prepn. from the cytoplasmic fraction of the leaf, triphosphopyridine nucleotide (TPN), pyruvate and manganous ion, fixed CO2 with a simultaneous evolution of oxygen. In expts. with tracer carbon the photosynthetic product was identified by paper chromatography and radioautography. No measurable CO2 fixation could be detected when one of the following was omitted: light, TPN, malic enzyme.