Multifunctional apparatus for electrokinetic processing of proteins
- 1 January 1994
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 15 (1) , 968-971
- https://doi.org/10.1002/elps.11501501141
Abstract
New equipment (the “Gradiflow TM”) has been designed and constructed to provide efficient large‐scale preparative fractionation of macromolecules, based on charge and/or size differences, as well as the concentration of macromolecules and electrodialysis. Examples of its capability are the separation of a mixture of haemoglobin (50 mg) from bovine serum albumin (50 mg) within 15 min (based on charge differences at pH 6.8), the purification of phycoerythrin from a crude extract on the basis of size, and the fractionation of serum proteins into two discrete size classes.Keywords
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