An Agrobacterium -transformed cell culture from the monocot Asparagus officinalis

Open Access

20 December 1984

journal article
research article
Published by Springer Nature in The EMBO Journal

Vol. 3 (13) , 3039-3041
https://doi.org/10.1002/j.1460-2075.1984.tb02254.x

Abstract

Cultured stem fragments from the monocotyledonous plant Asparagus officinalis infected by the oncogenic bacterium Agrobacterium tumefaciens developed tumorous proliferations. This tissue was propagated in vitro on hormone‐free culture medium. The T‐DNA‐encoded markers nopaline and agrocinopine were unambiguously detected in these tissues. The data demonstrate that stable T‐DNA transfer as well as expression of T‐DNA genes is possible in at least some monocotyledonous plants. This opens new possibilities for plant genetic engineering using the Ti plasmid as a gene vector.

Keywords

This publication has 11 references indexed in Scilit:

Expression of foreign genes in regenerated plants and in their progeny
The EMBO Journal, 1984
Inheritance of Functional Foreign Genes in Plants
Science, 1984
Characterization of the Enzyme Responsible for Nopaline and Ornaline Synthesis in Sunflower Crown Gall Tissues
Plant Physiology, 1978
A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities
Biochimica et Biophysica Acta (BBA) - Enzymology, 1978
Octopine and nopaline dehydrogenases in crown-gall tumors
Plant Science Letters, 1977
Octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciens
Molecular Genetics and Genomics, 1976
Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability
Nature, 1974
The loss of tumor-initiating ability inAgrobacterium tumefaciens by incubation at high temperature
Cellular and Molecular Life Sciences, 1971
Organic Growth Factor Requirements of Tobacco Tissue Cultures
Physiologia Plantarum, 1965
A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
Physiologia Plantarum, 1962