Phospholipid/Calcium-Dependent Protein Kinase Activity in Human Cortisol-Hypersecreting Adrenocortical Adenoma

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Abstract
We examined resected adrenocortical adenoma and adjacent atrophic adrenal tissue from a patient with Cushing's syndrome for phospholipid/calcium-dependent protein kinase (C-kinase) activity. Cytosolic C-kinase activity was higher in adrenocortical adenoma than in adjacent adrenal tissue, while C-kinase activity in the particulate fraction was lower in adrenocortical adenoma than in adjacent adrenal tissue. Upon stimulation of isolated adrenocortical adenoma cells with β 1-24 ACTH (10-7M), C-kinase activity in the cytosolic fraction was increased from 7pmol/min/mg protein (basal) to 107pmol/min/mg protein by 120min after stimulation. On the other hand, C-kinase activity in the particular fraction of the isolated cells was considerably decreased from 884pmol/min/mg protein (basal) to 179pmol/min/mg protein over the same period. These results suggest that C-kinase activity of adrenocortical adenoma cells was translocated from the particulate to the cytosolic fraction by ACTH-stimulation.

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