Tissue Bis(2-ethylhexyl)phthalate Levels in Uremic Subjects

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Abstract
A method for the analysis of tissue DEHP levels was developed. Tissue homogenates were extracted with a chloroformrmethanol solution, followed by the addition of 1 g baked alumina to clean up the heavy matrices of the tissues. DEHP levels in tne tissues were determined by gas chromatography. Percent recovery of DEHP from the tissues ranged between 72.2 and 83.3%. An experimentally produced acute renal failure in dogs (performing bilateral nephrectomy) was used for comparison of DEHP distribution in tissues from Control, Sham-Operated and Nephrectomized dogs. The highest concentration of DEKP was found in lung tissues of all three groups. DEHP levels in tissues of Nephrectomized dogs were significantly higher than those of Control and Sham-Operated dogs. With the exception of brain and liver, no significant difference in tissue DEHP levels were noted for Control and Sham-Operated dogs. Liver DEHP levels were 39.4 and 65.4 μg/g tissue for the Control and Sham-Operated dogs, respectively. DEHP was found in various tissues of some, but not all of the subjects who received hemodialysis treatments, blood transfusions or blood which was previously in contact with PVC. No sufficient information is available at the present time to draw a relationship between exposure to DEHP and unchanged DEHP content in the tissues. However, DEHP does appear to accumulate in tissues and could be detected at death from some, but not all patients. It is more likely, however, that DEHP undergoes rapid metabolism in tissues.