Determination of selenium in biological samples by hydride generation and atomic-absorption spectroscopy

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Abstract
Hydride generation followed by atomic-absorption spectroscopy has been used to determine the concentrations of selenium in 100-µl volumes of whole blood, plasma and erythrocyte suspensions following digestion of the samples with nitric and sulphuric acids. Analyses of human and animal sera agreed well with those obtained by other workers for selenium concentrations in the range 6–160 µg l–1. Relative standard deviations of 4.0–4.5% were obtained for replicate analyses of human plasma and whole blood containing selenium at concentrations of 120–144 µg l–1.