Human chorionic gonadotropin α and human cytomegalovirus promoters are extremely active in the fission yeast Schizosaccharomyces pombe
- 30 July 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 268 (1) , 217-221
- https://doi.org/10.1016/0014-5793(90)81012-d
Abstract
We have investigated the transcriptional activity of human cytomegalovirus, herpes thymidine kinase, human chorionic gonadotropin α, somatostatin, immunoglobulin κ chain, α crystallin, albumin and interferon-β promoters in the fission yeast Schizosaccharomyces pombe. Among these, the human cytomegalovirus, human chorionic gonadotropin α, and somatostatin promoters were found to be very active, approximately 11-, 9-, and 0.9-fold as active as the SV40 early promoter, respectively. The remainder of the promoters studied were weak, having only 10–20% of the SV40 promoter activity. Primer extension analysis showed that the strong promoters initiated transcription in S. pombe at the same sites as in mammalian cells, indicating the high similarity between both transcriptional systems.Keywords
This publication has 32 references indexed in Scilit:
- Isolation of the gene encoding the yeast TATA binding protein TFIID: A gene identical to the SPT15 suppressor of Ty element insertionsCell, 1989
- Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA boxNature, 1989
- Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding ProteinsScience, 1989
- Expression of the SV40 promoter in fission yeast: Identification and characterization of an AP-1-like factorCell, 1988
- A yeast and a human CCAAT-binding protein have heterologous subunits that are functionally interchangeableCell, 1988
- Transcriptional activation by the SV40 AP-1 recognition element in yeast is mediated by a factor similar to AP-1 that is distinct from GCN4Cell, 1988
- Synergism between immunoglobulin enhancers and promotersNature, 1986
- Evolutionary divergence of the mRNA transcription initiation mechanism in yeastNature, 1983
- Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: Identification of an upstream control regionCell, 1981
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976