Catalytic consequences of experimental evolution. Part 2. Rate-limiting degalactosylation in the hydrolysis of aryl β-D-galactopyranosides by the experimental evolvants ebg^aand ebg^b

1 January 1983

journal article
Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Perkin Transactions 2

No. 3,p. 365-369
https://doi.org/10.1039/p29830000365

Abstract

The build-up of a galactosyl-enzyme intermediate in the hydrolysis of 2-naphthylgalactoside by ebg^b enzyme has been observed by fluorescence in a stopped-flow apparatus. Degalactosylation has been shown to be rate limiting in the steady-state hydrolysis of 3,4-dinitrophenylgalactoside by both ebg^a and ebg^b enzymes by nucleophilic competition with methanol. The implications of this slowing down of degalactosylation as a consequence of experimental evolution of the gene are discussed.

Keywords

EXPERIMENTAL EVOLUTION
RATE LIMITING

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Catalytic consequences of experimental evolution. Part 2. Rate-limiting degalactosylation in the hydrolysis of aryl β-D-galactopyranosides by the experimental evolvants ebgaand ebgb

Abstract

Keywords

Catalytic consequences of experimental evolution. Part 2. Rate-limiting degalactosylation in the hydrolysis of aryl β-D-galactopyranosides by the experimental evolvants ebg^aand ebg^b