Localization and nucleotide sequence of the gene for the 8 kDa subunit of photosystem I in pea and wheat chloroplast DNA
- 1 January 1988
- journal article
- research article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 11 (3) , 311-319
- https://doi.org/10.1007/bf00027388
Abstract
The gene for the 8 kDa subunit of photosystem I has been located in the small single copy region of wheat chloroplast DNA by coupled transcription-translation of cloned fragments of DNA and by DNA sequence analysis. The pea gene for this subunit was located in pea chloroplast DNA by using the wheat gene as a probe. The location was confirmed by immunoprecipitation of the products of coupled transcription-translation of cloned DNA with antiserum raised against the small subunits of pea photosystem I and by DNA sequence analysis. The deduced amino acid sequences of the pea and wheat proteins are identical in seventy-six out of the eighty-one amino acid residues. There are nine conserved cysteine residues, eight of which are arranged in the primary structure in a similar way to those in bacterial ferredoxins containing two 4Fe-4S centres, suggesting that the polypeptide binds iron sulphur centres A and B of photosystem I.This publication has 38 references indexed in Scilit:
- N-terminal amino acid sequence analysis of the subunits of pea photosystem IFEBS Letters, 1988
- Tertiary structure of Bacillus thermoproteolyticus [4Fe-4S] ferredoxinJournal of Molecular Biology, 1988
- The 8 kDa polypeptide in photosystem I is a probable candidate of an iron‐sulfur center protein coded by the chloroplast gene frxAFEBS Letters, 1987
- Synthesis of a Photosystem I Polypeptide of 15 Kilodaltons in Isolated Etiochloroplasts of WheatPlant Physiology, 1986
- Photosystem I charge separation in the absence of centers A and B. I. Optical characterization of center ‘A2’ and evidence for its association with a 64-kDa peptideBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1986
- Tentative identification of the apoproteins of iron‐sulfur centers of Photosystem IFEBS Letters, 1984
- P700 Chlorophyll a-ProteinPlant Physiology, 1983
- On the interaction and orientation of the iron-sulfur centers A and B in chloroplasts of higher plantsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1981
- Site of synthesis of subunits to photosystem I reaction center and the proton—ATPase in SpirodelaFEBS Letters, 1981
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970