Determination of Trimeprazine and Its Main Metabolites in Mouse Serum and Thyroid by Liquid Chromatography-Electrospray-Mass Spectrometry

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Abstract
A sensitive and highly specific method for the determination of a phenothiazine neuroleptic, trimeprazine, and three of its main metabolites in mouse serum and thyroid, using combined liquid chromatography-electrospray-mass spectrometry (LC-ES-MS) was developed. A Nucleosil C18 (150 × 1 mm I.D.) reversed-phase column was used for the chromatographic separation together with a mixture of 5 mM ammonium formate buffer (pH 3) and acetonitrile (53/47; v/v) as mobile phase. Recoveries of the four analytes in serum were better than 60%, detection limits were 0.4 ng/mL, and limits of quantitation were 0.5 ng/mL for all analytes. Intra-assay precision, was close to 3% at 10 ng/mL and better than 18% at 2 ng/mL for the four analytes. Reproducibility was good (C.V. < 20%) and linearity excellent (r > 0.997) from 0.5 to 40 ng/mL. This sensitive technique allows the determination of serum and thyroid concentration of trimeprazine and metabolites in small tissue or serum samples.

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