Low-molecular-weight RNAs of Moloney murine leukemia virus: identification of the primer for RNA-directed DNA synthesis

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Abstract
The small RNAs of Moloney murine leukemia virus (M-MuLV) were fractionated into at least 15 species by 2-dimensional polyacrylamide gel electrophoresis. The pattern of small RNAs is significantly different from that of Rous sarcoma virus. A subset of the virion small RNAs is associated with the genome RNA in the 70S complex. One of the associated molecules, a cellular [mouse fibroblast 3T3, chicken embryo fibroblast and newborn rat kidney NRK cells] tRNA, is tightly bound to the genome RNA and serves as the major primer for M-MuLV RNA-directed DNA synthesis in vitro.