SOME EFFECTS OF CARBON SOURCE, AERATION, AND TEMPERATURE ON GROWTH OF A PSYCHROPHILIC STRAIN OF PSEUDOMONAS FLUORESCENS

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Abstract
A psychrophilic strain of Pseudomonas fluorescens was grown in stationary and aerated culture at 4, 10, 15, 20, 25 and 32 C. Generation times were determined for cultures growing in media containing glucose, monosodium-citrate or casamino-acids as the primary carbon and energy source. The results indicated that growth on casamino-acids with aeration contributed to significantly shorter generation times at low temperatures when compared with other media systems. Furthermore, temperature coefficients (Q10 values) calculated from growth-velocity data showed that the effects of temperature on growth rate are influenced by the carbon source in the growth medium and by aeration.