Molecular Cloning of an Amylase Gene ofBacillus circulans
- 1 June 1987
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA
- Vol. 6 (3) , 255-265
- https://doi.org/10.1089/dna.1987.6.255
Abstract
An amylase gene of Bacillus circulans was cloned in B. subtilis and its nucleotide sequence was determined. The putative proamylase consists of 528 amino acids, which correspond to a molecular weight of 58,776. Homologous regions with other amylases of Bacillus species were found. A σ55-type promoter is located at about 250 bp upstream from the starting codon. This promoter was also functional in Escherichia coli, and able to express β-galactosidase activity.Keywords
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