Embryonic stem cell–specific microRNAs promote induced pluripotency
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- 12 April 2009
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 27 (5) , 459-461
- https://doi.org/10.1038/nbt.1535
Abstract
Judson et al. show that microRNAs specific to mouse embryonic stem cells can substitute for the reprogramming factor cMyc in the generation of induced pluripotent stem cells. The development of reprogramming methods that do not rely on transgenes may facilitate clinical translation of this technology. This report demonstrates that introduction of microRNAs (miRNAs) specific to embryonic stem cells enhances the production of mouse induced pluripotent stem (iPS) cells. The miRNAs miR-291-3p, miR-294 and miR-295 increase the efficiency of reprogramming by Oct4, Sox2 and Klf4, but not by these factors plus cMyc. cMyc binds the promoter of the miRNAs, suggesting that they are downstream effectors of cMyc during reprogramming. However, unlike cMyc, the miRNAs induce a homogeneous population of iPS cell colonies.Keywords
This publication has 18 references indexed in Scilit:
- Embryonic stem cell–specific microRNAs regulate the G1-S transition and promote rapid proliferationNature Genetics, 2008
- Connecting microRNA Genes to the Core Transcriptional Regulatory Circuitry of Embryonic Stem CellsCell, 2008
- Integration of External Signaling Pathways with the Core Transcriptional Network in Embryonic Stem CellsCell, 2008
- miRiad Roles for the miR-17-92 Cluster in Development and DiseaseCell, 2008
- A mammalian microRNA cluster controls DNA methylation and telomere recombination via Rbl2-dependent regulation of DNA methyltransferasesNature Structural & Molecular Biology, 2008
- Defining Molecular Cornerstones during Fibroblast to iPS Cell Reprogramming in MouseCell Stem Cell, 2008
- Sequential Expression of Pluripotency Markers during Direct Reprogramming of Mouse Somatic CellsCell Stem Cell, 2008
- Generation of Induced Pluripotent Stem Cells in the Absence of Drug SelectionCell Stem Cell, 2007
- Genome-wide maps of chromatin state in pluripotent and lineage-committed cellsNature, 2007
- Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined FactorsCell, 2006