Conversion in vivo of injected estradiol-17β-4-14C and estrone-4-14C to urinary estradiol-17α-14C by the hen

Publisher Website
Google Scholar
Abstract
Radioactive estradiol-17α was identified in urine of hens that had received injections of estradiol-4-14C, or of estrone-4-14C, or of a mixture thereof. Identification was based on thin-layer chromatographic mobilities of the phenol and three derivatives, and by recrystallization of the phenol and of its diacetate in admixture with appropriate carrier to constant specific activity. The possibility of microbiological formation of the radioactive estradiol-17α after excretion of the urine was excluded by ad hoc experimentation. The results are advanced in support of the view that estradiol-17α is one of the normal urinary steroid estrogens of the fowl.

This publication has 0 references indexed in Scilit: