THE ACCUMULATION OF MYOINOSITOL AND RUBIDIUM IONS IN GALACTOSE-EXPOSED RAT LENS
- 1 October 1986
- journal article
- research article
- Vol. 27 (10) , 1522-1526
Abstract
When rat lens incubated at 30 mM galactose overnight, the extent of accumulation of rubidium ions (Rb) and myoinositol (MI) are affected, as well as the Na-K ATPase activity. Rb accumulation and Na-K ATPase activity are only slightly affected compared to the dramatic drop in MI accumulation. These changes are completely abolished by sorbinil, which blocks polyol formation, or by rendering the galactose medium hypertonic to offset the osmotic effect of polyol formation. On the other hand, the addition of excess MI to the galactose medium had no effect on correcting these changes. The results obtained are consistent with the polyol-osmotic theory of sugar cataract formation.This publication has 3 references indexed in Scilit:
- Prevention of defects of axonal transport and nerve conduction velocity by oral administration of myo-inositol or an aldose reductase inhibitor in streptozotocin-diabetic ratsDiabetologia, 1983
- Osmotic Effects on the Amino Acid-concentrating Mechanism in the Rabbit LensJournal of Biological Chemistry, 1965
- Studies on sodium-potassium-activated adenosinetriphosphatase. VI. Its role in cation transport in the lens of cat, calf and rabbitArchives of Biochemistry and Biophysics, 1963