The influence of normal and cancerous blood-serum on pancreatic lipase action and the effect of ionic and colloidal lead

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Abstract
The previous work on the influence of various compounds on pancreatic lipase is described briefly, more particularly the contributions of Shaw-Mackenzie and Rosenheim. Experiments carried out on the influence of filtration on the activity of the glycerol extract of lipase employed, and also on the effect of the order of mixing of the reactants, showed that (a) filtration of the glycerol extract through filter paper has no effect upon its activity, but the activity of the filtrate is destroyed by filtering through asbestos or a Jena Glass filter, (b) The order of mixing of the reactants is of great importance in deciding the results of experiments. Employing purified olive oil, the influence of Na oleate on olive oil (and also triacetin) hydrolysis by lipase was investigated. It was found that increasing concentrations of Na oleate caused an increase in the extent of hydrolysis in both cases, the augmentation by Na oleate rising to a maximum and subsequently falling with increasing concentration of Na oleate. Comparison of the augmentations produced by normal and cancerous human serum showed that cancerous serum possesses a reduced lipolytic augmentation as compared with normal serum, thus confirming the conclusions of Shaw-Mackenzie. Pb in both the ionic and colloidal form increases the augmentations produced by both normal and cancerous sera, when the Pb compounds were present in high and low concentrations respectively, i.e., in amounts both sufficient and insufficient to cause a precipitate with serum.

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