Interleukin‐1β antagonizes phenobarbital induction of several major cytochromes P450 in adult rat hepatocytes in primary culture
- 12 June 1995
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 366 (2-3) , 159-164
- https://doi.org/10.1016/0014-5793(95)00513-9
Abstract
We have investigated the effects of interleukin (IL)-1β and IL6 on expression and phenobarbital (PB) induction of ethoxyresorufin O-deethylase (EROD) and pentoxyresorufin O-deethylase (PROD) activities, as well as on mRNA levels of cytochromes P450 (CYP) 1A, 2B, 2C, 2E and 3A, in rat hepatocytes in primary culture. IL6 slightly antagonized PB-induced PROD activity. Strikingly, IL1β strongly inhibited basal EROD and PROD activities, and fully blocked their induction by PB in a dose-dependent fashion. Furthermore IL1β completely suppressed PB induction of all CYP mRNAs analyzed. Our results demonstrate that IL1β can suppress basal CYP activities, as well as PB-inducible expression of five CYP mRNAs in rat hepatocytes in primary cultureKeywords
This publication has 36 references indexed in Scilit:
- Recombinant human interleukin‐6 (IL‐6/BSF‐2/HSF) regulates the synthesis of acute phase proteins in human hepatocytesPublished by Wiley ,2001
- Caffeine Biotransformation in Human Hepatocyte Lines Derived from Normal Liver TissueBiochemical and Biophysical Research Communications, 1994
- Interleukin 4 inhibits the production of some acute‐phase proteins by human hepatocytes in primary cultureFEBS Letters, 1993
- Interleukin-6 down regulates the expression of transcripts encoding cytochrome P450 IA1, IA2 and IIIA3 in human hepatoma cellsBiochemical and Biophysical Research Communications, 1992
- Viability and primary culture of rat hepatocytes after hypothermic preservation: The superiority of the leibovitz medium over the university of wisconsin solution for cold storageHepatology, 1992
- Effect of interleukin 6 on phenobarbital induction of cytochrome P-450IIB in cultured rat hepatocytesBiochemical and Biophysical Research Communications, 1991
- Effect of interleukin 1 (IL-1) on the levels of cytochrome P-450 involving IL-1 receptor on the isolated hepatocytes of ratBiochemical and Biophysical Research Communications, 1990
- Phenobarbital, dexamethasone and benzanthracene induce several cytochrome P450 mRNAs in rat hepatoma cellsFEBS Letters, 1988
- Cytochrome P450 induction by phenobarbital (PB) is inhibited by 12-O-tetradecanoylphorbol-13-acetate(TPA): Evidence that protein kinase C regulates inductionBiochemical and Biophysical Research Communications, 1988
- Differential effects of phenobarbitone and 3-methylcholanthrene induction on the hepatic microsomal metabolism and cytochrome P-450-binding of phenoxazone and a homologous series of its n-alkyl ethers (alkoxyresorufins)Chemico-Biological Interactions, 1983